Polypeptide synthesis using an expressed peptide as a building block via the thioester method

Toru Kawakami, Koki Hasegawa, Kenta Teruya, Kenichi Akaji, Masataka Horiuchi, Fuyuhiko Inagaki, Yasuyuki Kurihara, Seiichi Uesugi, Saburo Aimoto

研究成果: Article

8 引用 (Scopus)

抜粋

An expressed peptide was proven to be useful as a building block in the synthesis of a polypeptide via the thioester method. A partially protected peptide segment, [Lys(Boc)15,25,31,48,57,68,80,95]- Max(14-101), was prepared via transamination of an N-terminal amino group from p21Max(13-101), which was obtained by expression using Escherichia coli. This peptide was utilized in the synthesis of [Ser(PO3H2)2,11]- p21Max(1-101) via condensation with the peptide thioester, Boc- [Ser(PO3H2)2,11]-p21Max(1-13)-SCH2CH2CO-Leu-OH. (C) 2000 Elsevier Science Ltd.

元の言語English
ページ(範囲)2625-2628
ページ数4
ジャーナルTetrahedron Letters
41
発行部数15
DOI
出版物ステータスPublished - 2000 4 8

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

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  • これを引用

    Kawakami, T., Hasegawa, K., Teruya, K., Akaji, K., Horiuchi, M., Inagaki, F., Kurihara, Y., Uesugi, S., & Aimoto, S. (2000). Polypeptide synthesis using an expressed peptide as a building block via the thioester method. Tetrahedron Letters, 41(15), 2625-2628. https://doi.org/10.1016/S0040-4039(00)00232-X