Polymer microbeads bound to c3 fragments for detecting and labeling cells with c3 receptors

Takafumi Uchida, S. Hosaka, Y. Murao

研究成果: Article査読

17 被引用数 (Scopus)

抄録

Monodisperse microbeads (mbs) of a hydrophilic polymer with diameters of 4ym and 0.2μym were prepared. To these mbs, IgM or a sugar was bound covalently, and then the mbs were treated with fresh human or mouse serum. This led to the activation of complement and to the binding of C3 fragments to the mbs. C3 fragments fixed on mbs varied with the type of immobilized substance on the mbs and the incubation time of the mbs with fresh serum. Three kinds of mbs binding C3 fragments were prepared : mbs binding C3b, C3bi and C3d (C3hu -mbs); the ones binding C3bi and C3d (C3mu-mbs); and the ones binding only C3b (C3hu-mbs). They formed rosettes with cells which had the corresponding receptor. When lymphocytes were incubated with C3hu-or C3hu-mbs with a diameter of 0.2μm, cap formation of each receptor was observed under a microscope. Once prepared, C3hu-, C3mu-and C3hu-mbs were stable for more than 4 months.

本文言語English
ページ(範囲)467-476
ページ数10
ジャーナルImmunological Investigations
11
6
DOI
出版ステータスPublished - 1982 1 1
外部発表はい

ASJC Scopus subject areas

  • Immunology

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