TY - JOUR
T1 - Pochoniolides A and B, new antioxidants from the fungal strain Pochonia chlamydosporia var. spinulospora FKI-7537
AU - Miyano, Rei
AU - Matsuo, Hirotaka
AU - Nonaka, Kenichi
AU - Mokudai, Takayuki
AU - Niwano, Yoshimi
AU - Shiomi, Kazuro
AU - Takahashi, Yōko
AU - Ōmura, Satoshi
AU - Nakashima, Takuji
N1 - Funding Information:
The authors declare no conflicts of interest. This study was supported by funds from the Institute for Fermentation, Osaka (IFO) , Japan.
Publisher Copyright:
© 2018 The Society for Biotechnology, Japan
PY - 2018/11
Y1 - 2018/11
N2 - New natural products, designated pochoniolides A and B, were isolated from the cultured broth of fungal strain FKI-7537 using a physicochemical screening methodology. Strain FKI-7537 was isolated from a soil sample collected at Niijima, Tokyo, Japan and identified as Pochonia chlamydosporia var. spinulospora by morphological characteristics and DNA sequence analysis. The chemical structures of pochoniolides A and B were elucidated by NMR and mass spectra and found to be new compounds consisting of a muconolactone moiety connected with a chromone unit. Pochoniolides A and B were identified as racemate mixtures using data on optical rotation and circular dichroism spectra. Furthermore, enantiomers of pochoniolide B, pochoniolides B1 and B2, were separated using a chiral HPLC column. Pochoniolides A and B showed hydroxyl radical-scavenging and singlet oxygen-quenching activities.
AB - New natural products, designated pochoniolides A and B, were isolated from the cultured broth of fungal strain FKI-7537 using a physicochemical screening methodology. Strain FKI-7537 was isolated from a soil sample collected at Niijima, Tokyo, Japan and identified as Pochonia chlamydosporia var. spinulospora by morphological characteristics and DNA sequence analysis. The chemical structures of pochoniolides A and B were elucidated by NMR and mass spectra and found to be new compounds consisting of a muconolactone moiety connected with a chromone unit. Pochoniolides A and B were identified as racemate mixtures using data on optical rotation and circular dichroism spectra. Furthermore, enantiomers of pochoniolide B, pochoniolides B1 and B2, were separated using a chiral HPLC column. Pochoniolides A and B showed hydroxyl radical-scavenging and singlet oxygen-quenching activities.
KW - Antioxidant
KW - Muconolactone
KW - Physicochemical screening
KW - Pochonia chlamydosporia var. spinulospora
KW - Pochoniolides
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U2 - 10.1016/j.jbiosc.2018.05.003
DO - 10.1016/j.jbiosc.2018.05.003
M3 - Article
C2 - 29941346
AN - SCOPUS:85048869291
VL - 126
SP - 661
EP - 666
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 5
ER -