PIH1D1 interacts with mTOR complex 1 and enhances ribosome RNA transcription

Yuya Kamano, Makio Saeki, Hiroshi Egusa, Yoshito Kakihara, Walid A. Houry, Hirofumi Yatani, Yoshinori Kamisaki

研究成果: Article査読

12 被引用数 (Scopus)

抄録

PIH1D1 is the defining component of the R2TP complex. Recently, R2TP has been reported to stabilize mTOR (mammalian target of rapamycin), an important regulator of cell growth and protein synthesis. Two complexes of mTOR, mTORC1 and mTORC2, have been identified. We demonstrate that immunoprecipitation (IP) of PIH1D1 results in the co-IP of Raptor (mTORC1 specific), but not Rictor (mTORC2 specific), and that knockdown of PIH1D1 decreases mTORC1 assembly, S6 kinase phosphorylation (indicator of mTORC1 activity), and rRNA transcription without affecting mTORC2 in human breast cancer MCF-7 cells. In addition, we provide evidence that PIH1D1 is overexpressed in various breast cancer cell lines. These findings collectively suggest that PIH1D1 may have an important role in mTORC1 regulation in breast cancers.

本文言語English
ページ(範囲)3303-3308
ページ数6
ジャーナルFEBS Letters
587
20
DOI
出版ステータスPublished - 2013 10 11
外部発表はい

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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