Phylogenetic analysis of saccharolytic oral treponemes isolated from human subgingival plaque

Mitsuo Sakamoto, Takeyoshi Koseki, Makoto Umeda, Isao Ishikawa, Yoshimi Benno, Takashi Nakase

研究成果: Article査読

6 被引用数 (Scopus)

抄録

A total of 74 strains of oral treponemes, which were isolated from subgingival plaque samples from patients with periodontitis, were taxonomically studied on the basis of biochemical characteristics, DNA-DNA hybridization, and 16S rRNA gene sequences. These organisms fermented carbohydrates and required rumen fluid or short-chain volatile fatty acids for growth. The isolates were divided into seven subgroups based on their biochemical characteristics. The levels of DNA relatedness among the representative strains of each subgroup and Treponema socranskii (including three subspecies) were greater than 78%, while the levels of DNA relatedness among these strains and other Treponema species, including T. denticola and 'T. vincentii', were less than 15%, DNA-DNA hybridization indicated that all subgroups belonged to T. socranskii. This result correlated well with the cluster on the phylogenetic trees based on 16S rRNA sequences.

本文言語English
ページ(範囲)711-716
ページ数6
ジャーナルMICROBIOLOGY and IMMUNOLOGY
43
7
DOI
出版ステータスPublished - 1999
外部発表はい

ASJC Scopus subject areas

  • 微生物学
  • 免疫学
  • ウイルス学

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