Photothermal imaging of skeletal muscle mitochondria

Toru Tomimatsu, Jun Miyazaki, Yutaka Kano, Takayoshi Kobayashi

研究成果: Article査読

5 被引用数 (Scopus)


The morphology and topology of mitochondria provide useful information about the physiological function of skeletal muscle. Previous studies of skeletal muscle mitochondria are based on observation with transmission, scanning electron microscopy or fluorescence microscopy. In contrast, photothermal (PT) microscopy has advantages over the above commonly used microscopic techniques because of no requirement for complex sample preparation by fixation or fluorescent-dye staining. Here, we employed the PT technique using a simple diode laser to visualize skeletal muscle mitochondria in unstained and stained tissues. The fine mitochondrial network structures in muscle fibers could be imaged with the PT imaging system, even in unstained tissues. PT imaging of tissues stained with toluidine blue revealed the structures of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria and the swelling behavior of mitochondria in damaged muscle fibers with sufficient image quality. PT image analyses based on fast Fourier transform (FFT) and Grey-level co-occurrence matrix (GLCM) were performed to derive the characteristic size of mitochondria and to discriminate the image patterns of normal and damaged fibers.

ジャーナルBiomedical Optics Express
出版ステータスPublished - 2017 6月 1

ASJC Scopus subject areas

  • バイオテクノロジー
  • 原子分子物理学および光学


「Photothermal imaging of skeletal muscle mitochondria」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。