Phospholipid polymer multilayered hydrogels containing cells for cancer drug screening

Kazuhiko Ishihara, Botao Gao, Tomohiro Konno

研究成果: Conference contribution

抄録

Statement of Purpose: Hydrogel multilayers with micrometer-scale in thickness containing living cancer cells were prepared by layer-by-layer assembling with 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer solution and poly(vinyl alcohol) (PVA) solution alternatively under biological conditions. It was demonstrated that this tool could be utilized for minimizing barriers to the diffusion of drugs while preserving the 3D context. As a case study, the cytotoxicity effects of paclitaxel (PTX) on human cervical cancer HeLa-Fucci cells were evaluated in vitro. A comparative study was carried out: 2D culture, 3D culture in microgel thin membrane and in macrogel. Time-lapse microscopic analyses showed HeLa-Fucci cells cultured on 2D exhibited a more rapid and robust response to stimulation than cells cultured in microgel thin membrane. Besides, HeLa-Fucci cells cultured under macrogel did not show any response to stimulation. It was speculated that both of the diffusion of PTX and matrix dimensionality could affect drug efficacy and diffusion of drug seemed to play a more important role.

本文言語English
ホスト出版物のタイトルSociety for Biomaterials Annual Meeting and Exposition 2019
ホスト出版物のサブタイトルThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
出版社Society for Biomaterials
ページ659
ページ数1
ISBN(電子版)9781510883901
DOI
出版ステータスPublished - 2019
外部発表はい
イベント42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
継続期間: 2019 4 32019 4 6

出版物シリーズ

名前Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
40
ISSN(印刷版)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
国/地域United States
CitySeattle
Period19/4/319/4/6

ASJC Scopus subject areas

  • 生化学
  • 生物理学
  • バイオテクノロジー
  • 生体材料
  • 材料化学

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