Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor

Yoshimichi Murata, Hirohide Iwasaki, Mari Sasaki, Kazuo Inaba, Yasushi Okamura

研究成果: Article査読

546 被引用数 (Scopus)

抄録

Changes in membrane potential affect ion channels and transporters, which then alter intracellular chemical conditions. Other signalling pathways coupled to membrane potential have been suggested but their underlying mechanisms are unknown. Here we describe a novel protein from the ascidian Ciona intestinalis that has a transmembrane voltage-sensing domain homologous to the S1-S4 segments of voltage-gated channels and a cytoplasmic domain similar to phosphatase and tensin homologue. This protein, named C. intestinalis voltage-sensor-containing phosphatase (Ci-VSP), displays channel-like 'gating' currents and directly translates changes in membrane potential into the turnover of phosphoinositides. The activity of the phosphoinositide phosphatase in Ci-VSP is tuned within a physiological range of membrane potential. Immunocytochemical studies show that Ci-VSP is expressed in Ciona sperm tail membranes, indicating a possible role in sperm function or morphology. Our data demonstrate that voltage sensing can function beyond channel proteins and thus more ubiquitously than previously realized.

本文言語English
ページ(範囲)1239-1243
ページ数5
ジャーナルNature
435
7046
DOI
出版ステータスPublished - 2005 6月 30
外部発表はい

ASJC Scopus subject areas

  • 一般

フィンガープリント

「Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル