Dendritic cells (DCs) are more effective antigen-presenting cells (APCs) than B cells and macrophages, and they also play important roles in innate and adaptive immune responses. DCs are derived from bone marrow and exist in all lymphoid and most nonlymphoid tissues. There are some differences in morphology, phenotypes, and functions between subtypes of DCs. However, the phenotypes and functions of bovine DCs are poorly understood. Therefore, we have tried to develop suitable methods to identify peripheral blood DCs using negative selection. Following the removal of T cells, B cells and monocytes/ macrophages by antibody-conjugated magnetic beads, we succeeded in the detection of blood DCs, which strongly expressed MHC II molecules, CD11c and the myeloid lineage marker CD172a by FACS analysis. These CD11c+/ CD172a+ DCs morphologically displayed monocyte-like features at the middle stages of maturation. We also detected the same phenotype cell population in the thymic medulla as blood DCs. In addition, we found new thymus specific phenotypes of bovine DCs, such as CD1 and CD172a double positive cells, though we did not find these in peripheral blood. We additionally investigated the distribution of CD172a positive cells in bovine intestinal Peyer’s patches. CD172a positive cells were found in the dome region of Peyer’s patches and interestingly some CD172a positive cells also expressed the prion protein (PrP), which is thought to be involved in the development of bovine spongiform encephalopathy (BSE). These results indicate the possibility that myeloid DCs may be involved in the transmission and replication of the BSE agent. With regard to the technical benefit, the methods that we have developed enable us to isolate a blood subset of bovine DC phenotypes from blood using a cell sorter and to investigate the functions and the interaction between DCs and other cell types.
|ホスト出版物のサブタイトル||Types, Life Cycles and Biological Functions|
|出版社||Nova Science Publishers, Inc.|
|出版ステータス||Published - 2010 1 1|
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