pH dependence of the fluorescence lifetime of FAD in solution and in cells

Md Serajul Islam, Masato Honma, Takakazu Nakabayashi, Masataka Kinjo, Nobuhiro Ohta

研究成果: Article査読

65 被引用数 (Scopus)

抄録

We have studied physiological parameters in a living cell using fluorescence lifetime imaging of endogenous chromophores. In this study, pH dependence of the fluorescence lifetime of flavin adenine dinucleotide (FAD), that is a significant cofactor exhibiting autofluorescence, has been investigated in buffer solution and in cells. The fluorescence lifetime of FAD remained unchanged with pH 5 to 9 in solution. However, the fluorescence lifetime in HeLa cells was found to decrease with increasing intracellular pH, suggesting that pH in a single cell can be estimated from the fluorescence lifetime imaging of FAD without adding exogenous fluorescent probes.

本文言語English
ページ(範囲)1952-1963
ページ数12
ジャーナルInternational journal of molecular sciences
14
1
DOI
出版ステータスPublished - 2013 1
外部発表はい

ASJC Scopus subject areas

  • 触媒
  • 分子生物学
  • 分光学
  • コンピュータ サイエンスの応用
  • 物理化学および理論化学
  • 有機化学
  • 無機化学

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