PH dependence of the fluorescence lifetime of enhanced yellow fluorescent protein in solution and cells

Takakazu Nakabayashi, Shugo Oshita, Ryoya Sumikawa, Fan Sun, Masataka Kinjo, Nobuhiro Ohta

研究成果: Article査読

10 被引用数 (Scopus)

抄録

pH dependence of the fluorescence decay profile of enhanced yellow fluorescent protein (EYFP) depends on the excitation wavelength. The correlation between the fluorescence lifetime and pH in solution is discussed in terms of the acid-base equilibrium of the chromophore of EYFP. Fluorescence lifetime images of EYFP in HeLa cells have also been measured at various values of intracellular pH. A remarkable pH dependence of the fluorescence lifetime image is observed in pH 4.5-6.0, indicating that pH in a single cell can be evaluated using the fluorescence lifetime image of EYFP especially in the acidic condition.

本文言語English
ページ(範囲)65-71
ページ数7
ジャーナルJournal of Photochemistry and Photobiology A: Chemistry
235
DOI
出版ステータスPublished - 2012 5 1
外部発表はい

ASJC Scopus subject areas

  • 化学 (全般)
  • 化学工学(全般)
  • 物理学および天文学(全般)

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