Permselectivity of voltage-gated alamethicin ion channel studied by microamperometry

Tomokazu Matsue; Hitoshi Shiku; Hiroshi Yamada; Isamu Uchida

doi:10.1021/j100094a002

Permselectivity of voltage-gated alamethicin ion channel studied by microamperometry

Tomokazu Matsue, Hitoshi Shiku, Hiroshi Yamada, Isamu Uchida

研究成果: Article › 査読

28 被引用数 (Scopus)

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The permeability of Ru(NH₃)₆³⁺, Fe(CN)₆^3-, and I^- through voltage-gated alamethicin ion channels in a planar lipid membrane was investigated by amperometric measurements using a microelectrode located near the membrane. The redox current at the microelectrode initiated by application of a potential pulse to the BLM was quantitatively analyzed to obtain permselectivity of the channel. The permeability of Fe(CN)₆^3- was ca. 10 times smaller than that of Ru(NH₃)₆³⁺ due to electrostatic repulsion, indicating that the channel gives a high barrier to the permeation of multicharged negative ions.

本文言語	English
ページ（範囲）	11001-11003
ページ数	3
ジャーナル	Journal of physical chemistry
巻	98
号	43
DOI	https://doi.org/10.1021/j100094a002
出版ステータス	Published - 1994 1月 1

ASJC Scopus subject areas

工学（全般）
物理化学および理論化学

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10.1021/j100094a002

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title = "Permselectivity of voltage-gated alamethicin ion channel studied by microamperometry",

abstract = "The permeability of Ru(NH3)63+, Fe(CN)63-, and I- through voltage-gated alamethicin ion channels in a planar lipid membrane was investigated by amperometric measurements using a microelectrode located near the membrane. The redox current at the microelectrode initiated by application of a potential pulse to the BLM was quantitatively analyzed to obtain permselectivity of the channel. The permeability of Fe(CN)63- was ca. 10 times smaller than that of Ru(NH3)63+ due to electrostatic repulsion, indicating that the channel gives a high barrier to the permeation of multicharged negative ions.",

author = "Tomokazu Matsue and Hitoshi Shiku and Hiroshi Yamada and Isamu Uchida",

year = "1994",

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AU - Matsue, Tomokazu

AU - Shiku, Hitoshi

AU - Yamada, Hiroshi

AU - Uchida, Isamu

PY - 1994/1/1

Y1 - 1994/1/1

N2 - The permeability of Ru(NH3)63+, Fe(CN)63-, and I- through voltage-gated alamethicin ion channels in a planar lipid membrane was investigated by amperometric measurements using a microelectrode located near the membrane. The redox current at the microelectrode initiated by application of a potential pulse to the BLM was quantitatively analyzed to obtain permselectivity of the channel. The permeability of Fe(CN)63- was ca. 10 times smaller than that of Ru(NH3)63+ due to electrostatic repulsion, indicating that the channel gives a high barrier to the permeation of multicharged negative ions.

AB - The permeability of Ru(NH3)63+, Fe(CN)63-, and I- through voltage-gated alamethicin ion channels in a planar lipid membrane was investigated by amperometric measurements using a microelectrode located near the membrane. The redox current at the microelectrode initiated by application of a potential pulse to the BLM was quantitatively analyzed to obtain permselectivity of the channel. The permeability of Fe(CN)63- was ca. 10 times smaller than that of Ru(NH3)63+ due to electrostatic repulsion, indicating that the channel gives a high barrier to the permeation of multicharged negative ions.

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Permselectivity of voltage-gated alamethicin ion channel studied by microamperometry

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