@inproceedings{4db46bd1a8dc40ca89f113441bdb7838,
title = "Perfusion culture through a vasculature constructed in a tumor spheroid",
abstract = "We developed a microfluidic system for a perfusion culture of tumor spheroids utilizing engineered vasculatures. By the definition of the cellular interaction between fibroblast cells in a tumor spheroid and endothelial cells in microchannels, we successfully induced angiogenic sprouts from the microchannels, which anastomosed with the spheroid and formed perfusable vasculatures. We continuously supplied medium to the tumor spheroid through the vasculature and evaluated the effect of perfusion on the growth of the tumor spheroid. Our perfusion system greatly improved the cellular proliferation activity, mimicking the nutrition supply and evacuation of waste product through vasculatures surrounding tumors in vivo.",
keywords = "Angiogenesis, Microfluidic device, Perfusable vasculature, Spheroid, Tissue culture",
author = "Yuji Nashimoto and Yukako Teraoka and Akiko Nakamasu and Sanshiro Hanada and Yuichiro Arima and Torisawa, {Yu Suke} and Hidetoshi Kotera and Koichi Nishiyama and Takashi Miura and Ryuji Yokokawa",
note = "Funding Information: This work was supported by CREST, Japan Science and Technology Agency (JST), JSPS KAKENHI Grant Number 16K16386, Mizuho Foundation for the Promotion of Sciences. We also thank Yazaki Memorial Foundation for Science and Technology for supporting the travel. Publisher Copyright: {\textcopyright} 17CBMS-0001.; 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 ; Conference date: 22-10-2017 Through 26-10-2017",
year = "2020",
language = "English",
series = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017",
publisher = "Chemical and Biological Microsystems Society",
pages = "1029--1030",
booktitle = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017",
}