Pelotomaculum thermopropionicum gen. nov., sp. nov., an anaerobic, thermophilic, syntrophic propionate-oxidizing bacterium

Hiroyuki Imachi, Yuji Sekiguchi, Yoichi Kamagata, Satoshi Hanada, Akiyoshi Ohashi, Hideki Harada

研究成果: Article査読

155 被引用数 (Scopus)

抄録

An anaerobic, thermophilic, syntrophic propionate-oxidizing bacterium, strain SIT, isolated previously from granular sludge in a thermophilic upflow anaerobic sludge blanket (UASB) reactor, was characterized. The strain could grow fermentatively on pyruvate and fumarate in pure culture. The strain grew on propionate, ethanol, lactate, 1-butanol, 1-pentanol, 1,3-propanediol, 1-propanol and ethylene glycol in co-culture with the hydrogenotrophic methanogen Methanothermobacter thermautotrophicus strain ΔHT . The optimum temperature for growth was 55 °C and the pH optimum was 7.0. The G+C content of the DNA was 52.8 mol %. Strain SIT contained MK-7 and MK-7(H4) as the major quinones and contained iso-C15:0 as the major fatty acid. Based on 16S rDNA sequence analysis, strain SIT formed a novel lineage within the Gram-positive, spore-forming, sulphate-reducing bacterial group Desulfotomaculum. However, the strain lacked the ability to conduct dissimilatory sulphate reduction. Instead, it could reduce fumarate to succinate with concomitant growth on several organic substances as electron donor. These phenotypic and genetic properties support the formation of a novel species of a new genus, for which the name Pelotomaculum thermopropionicum gen. nov., sp. nov. is proposed. The type strain is strain SIT (= DSM 13744T = JCM 10971T).

本文言語English
ページ(範囲)1729-1735
ページ数7
ジャーナルInternational journal of systematic and evolutionary microbiology
52
5
DOI
出版ステータスPublished - 2002 9 1

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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