Parallel electron donation pathways to cytochrome cz in the type I homodimeric photosynthetic reaction center complex of Chlorobium tepidum

Yusuke Tsukatani, Chihiro Azai, Toru Kondo, Shigeru Itoh, Hirozo Oh-oka

研究成果: Article査読

11 被引用数 (Scopus)

抄録

We studied the regulation mechanism of electron donations from menaquinol:cytochrome c oxidoreductase and cytochrome c-554 to the type I homodimeric photosynthetic reaction center complex of the green sulfur bacterium Chlorobium tepidum. We measured flash-induced absorption changes of multiple cytochromes in the membranes prepared from a mutant devoid of cytochrome c-554 or in the reconstituted membranes by exogenously adding cytochrome c-555 purified from Chlorobium limicola. The results indicated that the photo-oxidized cytochrome cz bound to the reaction center was rereduced rapidly by cytochrome c-555 as well as by the menaquinol:cytochrome c oxidoreductase and that cytochrome c-555 did not function as a shuttle-like electron carrier between the menaquinol:cytochrome c oxidoreductase and cytochrome cz. It was also shown that the rereduction rate of cytochrome cz by cytochrome c-555 was as high as that by the menaquinol:cytochrome c oxidoreductase. The two electron-transfer pathways linked to sulfur metabolisms seem to function independently to donate electrons to the reaction center.

本文言語English
ページ(範囲)1211-1217
ページ数7
ジャーナルBiochimica et Biophysica Acta - Bioenergetics
1777
9
DOI
出版ステータスPublished - 2008 9
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 細胞生物学

フィンガープリント

「Parallel electron donation pathways to cytochrome c<sub>z</sub> in the type I homodimeric photosynthetic reaction center complex of Chlorobium tepidum」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル