@article{995a34ef91c449bc87b147b63e07da96,
title = "Parallel and patterned optogenetic manipulation of neurons in the brain slice using a DMD-based projector",
abstract = "Optical manipulation technologies greatly advanced the understanding of the neuronal network and its dysfunctions. To achieve patterned and parallel optical switching, we developed a microscopic illumination system using a commercial DMD-based projector and a software program. The spatiotemporal patterning of the system was evaluated using acute slices of the hippocampus. The neural activity was optically manipulated, positively by the combination of channelrhodopsin-2 (ChR2) and blue light, and negatively by the combination of archaerhodopsin-T (ArchT) and green light. It is suggested that our projector-managing optical system (PMOS) would effectively facilitate the optogenetic analyses of neurons and their circuits.",
keywords = "Archaerhodopsin, Brain slice, Channelrhodopsin, Light stimulation, Microscope, Optics, Optogenetics, Software",
author = "Seiichiro Sakai and Kenichi Ueno and Toru Ishizuka and Hiromu Yawo",
note = "Funding Information: The authors gratefully thank Drs. Georg Nagel, Edward S. Boyden, Atsushi Miyawaki and Roger Y. Tsien for the cDNA plasmids and B. Bell for language assistance. This work was supported by Grants-in-aid for scientific research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan , Global COE Program (Basic & Translational Research Center for Global Brain Science) , MEXT, Strategic Research Program for Brain Sciences (SRPBS, MEXT) and by the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation (NIBIO) .",
year = "2013",
month = jan,
doi = "10.1016/j.neures.2012.03.009",
language = "English",
volume = "75",
pages = "59--64",
journal = "Neuroscience Research",
issn = "0168-0102",
publisher = "Elsevier Ireland Ltd",
number = "1",
}