Palmitoylated ras proteins traffic through recycling endosomes to the plasma membrane during exocytosis

Ryo Misaki, Miki Morimatsu, Takefumi Uemura, Satoshi Waguri, Eiji Miyoshi, Naoyuki Taniguchi, Michiyuki Matsuda, Tomohiko Taguchi

研究成果: Article査読

62 被引用数 (Scopus)

抄録

Ras proteins regulate cell growth, death, and differentiation, and it is well established that this functional versatility is accomplished through their different subcellular localizations. Palmitoylated H- and N-Ras are believed to localize at the perinuclear Golgi and plasma membrane (PM). Notably, however, recycling endosomes (REs) also localize to a perinuclear region, which is often indistinguishable from the Golgi. In this study, we show that active palmitoylated Ras proteins mainly localize intracellularly at REs and that REs act as a way station along the post-Golgi exocytic pathway to the PM. H-Ras requires two palmitoyl groups for RE targeting. The lack of either or both palmitoyl groups leads to the mislocalization of the mutant proteins to the endoplasmic reticulum, Golgi apparatus, or the PM. Therefore, we demonstrate that palmitoylation directs Ras proteins to the correct intracellular organelles for trafficking and activity.

本文言語English
ページ(範囲)23-29
ページ数7
ジャーナルJournal of Cell Biology
191
1
DOI
出版ステータスPublished - 2010 10 4
外部発表はい

ASJC Scopus subject areas

  • 細胞生物学

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