Oxysterol regulation of estrogen receptor α-mediated gene expression in a transcriptional activation assay system using HeLa cells

Hiroyoshi Sato, Satoko Nishida, Hiroko Tomoyori, Masao Sato, Ikuo Ikeda, Katsumi Imaizumi

研究成果: Article査読

20 被引用数 (Scopus)

抄録

In order to test the estrogenic activity of sterol oxidation products from cholesterol and phytosterols, an estrogen-dependent gene expression assay was performed in estrogen receptor α-stably transformed HeLa cells. The ranking of the estrogenic potency of these compounds was different: 17β-estradiol ≫ genistein ≫ β-epoxycholesterol = daidzein = cholestanetriol = 22(R)-hydroxycholesterol = 20(S)-hydroxycholesterol = sitostanetriol > campestanetriol = β-epoxysitosterol = 7β-hydroxycholesterol. These compounds were not estrogenic in estrogen receptor-negative HeLa cells.

本文言語English
ページ(範囲)1790-1793
ページ数4
ジャーナルBioscience, Biotechnology and Biochemistry
68
8
DOI
出版ステータスPublished - 2004 8月
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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