Overexpression of manganese-superoxide dismutase prevents methylmercury toxicity in hela cells

Akira Naganuma, Kyoko Miura, Toshiko Tanaka-Kagawa, Jun Kitahara, Yoshiyuki Seko, Haruka Toyoda, Nobumasa Imura

研究成果: Article査読

35 被引用数 (Scopus)

抄録

HeLa cells were stably transformed with plasmid constructs that allowed constitutive expression of antioxidant enzymes such as catalase, glutathione peroxidase (GSH-Px). Cu,Zn-superoxide dismutase (Cu,Zn-SOD) or Mn-superoxide dismutase (Mn-SOD) to examine the involvement of reactive oxygen generation in methylmercury toxicity. Overexpression of catalase, GSH-Px or Cu,Zn-SOD did not affect the sensitivity of HeLa cells against methylmercury. However, the sensitivity of HeLa cells against methylmercury was decreased by overexpression of Mn-SOD, an enzyme localized in matrix of mitochondria and which decomposes superoxide anions. These results suggest that formation of superoxide anions in the mitochondria might be involved in the mechanism of the cytotoxicity of methylmercury.

本文言語English
ジャーナルLife Sciences
62
12
DOI
出版ステータスPublished - 1998 2月 13

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 薬理学、毒性学および薬学(全般)

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