Osterix regulates calcification and degradation of chondrogenic matrices through matrix metalloproteinase 13 (MMP13) expression in association with transcription factor Runx2 during endochondral ossification

Riko Nishimura, Makoto Wakabayashi, Kenji Hata, Takuma Matsubara, Shiho Honma, Satoshi Wakisaka, Hiroshi Kiyonari, Go Shioi, Akira Yamaguchi, Noriyuki Tsumaki, Haruhiko Akiyama, Toshiyuki Yoneda

    研究成果: Article査読

    95 被引用数 (Scopus)

    抄録

    Endochondral ossification is temporally and spatially regulated by several critical transcription factors, including Sox9, Runx2, and Runx3. Although the molecular mechanisms that control the late stages of endochondral ossification (e.g. calcification) are physiologically and pathologically important, these precise regulatory mechanisms remain unclear. Here, we demonstrate that Osterix is an essential transcription factor for endochondral ossification that functions downstream of Runx2. The global and conditional Osterix-deficient mice studied here exhibited a defect of cartilage-matrix ossification and matrix vesicle formation. Importantly, Osterix deficiencies caused the arrest of endochondral ossification at the hypertrophic stage. Microarray analysis revealed that matrix metallopeptidase 13 (MMP13) is an important target of Osterix. We also showed that there exists a physical interaction between Osterix and Runx2 and that these proteins function cooperatively to induceMMP13during chondrocyte differentiation. Most interestingly, the introduction ofMMP13stimulated the calcification of matrices in Osterix-deficient mouse limb bud cells. Our results demonstrated that Osterix was essential to endochondral ossification and revealed that the physical and functional interaction between Osterix and Runx2 were necessary for the induction of MMP13 during endochondral ossification.

    本文言語English
    ページ(範囲)33179-33190
    ページ数12
    ジャーナルJournal of Biological Chemistry
    287
    40
    DOI
    出版ステータスPublished - 2012 9 28

    ASJC Scopus subject areas

    • 生化学
    • 分子生物学
    • 細胞生物学

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