Osteopontin is involved in migration of eosinophils in asthma

A. Takahashi, M. Kurokawa, S. Konno, K. Ito, S. Kon, S. Ashino, T. Nishimura, T. Uede, N. Hizawa, S. K. Huang, M. Nishimura

研究成果: Article査読

33 被引用数 (Scopus)

抄録

Background Osteopontin (OPN) is an extracellular matrix protein with a wide range of functions, and is involved in various inflammatory diseases. However, the role of OPN in eosinophilic airway inflammation is unclear. Objective To elucidate the role of OPN in eosinophilic airway inflammation. Methods OPN protein levels in induced sputum from asthmatic patients and healthy controls were measured. Eosinophil migration assays were performed in the presence or absence of OPN, a blocking antibody (Ab) recognizing its integrin-binding domain (2K1) and an anti-integrin α4 Ab (P1H4). In the mouse asthma model, the levels of eosinophilia were examined in bronchoalveolar lavage fluids (BALFs) from ovalbumin (OVA)-sensitized and -challenged mice with or without administration of an Ab (M5) corresponding to human 2K1. Results Levels of OPN in induced sputum were significantly higher in asthmatic patients when compared with healthy controls. In addition, levels of OPN were correlated with the percentage of sputum eosinophils. OPN induced significant migration of human eosinophils and this effect was inhibited by 2K1 and P1H4. M5 significantly attenuated OVA-induced eosinophilia in BALFs. Conclusion These results indicate that OPN plays a role in the migration of eosinophils into the airways and may be involved in the pathogenesis of asthma.

本文言語English
ページ(範囲)1152-1159
ページ数8
ジャーナルClinical and Experimental Allergy
39
8
DOI
出版ステータスPublished - 2009 8
外部発表はい

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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