Organic cation transporter 3 modulates murine basophil functions by controlling intracellular histamine levels

Elke Schneider, François Machavoine, Jean Marie Pléau, Anne France Bertron, Robin L. Thurmond, Hiroshi Ohtsu, Takehiko Watanabe, Alfred H. Schinkel, Michel Dy

研究成果: Article査読

67 被引用数 (Scopus)

抄録

In this study, we identify the bidirectional organic cation transporter 3 (OCT3/Slc22a3) as the molecule responsible for histamine uptake by murine basophils. We demonstrate that OCT3 participates in the control of basophil functions because exogenous histamine can inhibit its own synthesis-and that of interleukin (IL)-4, IL-6, and IL-13-through this means of transport. Furthermore, ligands of H3/H4 histamine receptors or OCT3 inhibit histamine uptake, and outward transport of newly synthesized histamine. By doing so, they increase the histamine content of basophils, which explains why they mimic the effect of exogenous histamine. These drugs were no longer effective in histamine-free histidine decarboxylase (HDC)-deficient mice, in contrast with histamine itself. Histamine was not taken up and lost its inhibitory effect in mice deficient for OCT3, which proved its specific involvement. Intracellular histamine levels were increased strongly in IL-3-induced OCT3-/- bone marrow basophils, and explained why they generated fewer cytokines than their wild-type counterpart. Their production was enhanced when histamine synthesis was blocked by the specific HDC inhibitor α-fluoro-methyl histidine, and underscored the determinant role of histamine in the inhibitory effect. We postulate that pharmacologic modulation of histamine transport might become instrumental in the control of basophil functions during allergic diseases.

本文言語English
ページ(範囲)387-393
ページ数7
ジャーナルJournal of Experimental Medicine
202
3
DOI
出版ステータスPublished - 2005 8 1

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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