Orexin-A in the human brain and tumor tissues of ganglioneuroblastoma and neuroblastoma

Zenei Arihara, Kazuhiro Takahashi, Osamu Murakami, Kazuhito Totsune, Masahiko Sone, Fumitoshi Satoh, Sadayoshi Ito, Yutaka Hayashi, Hironobu Sasano, Toraichi Mouri

研究成果: Article査読

50 被引用数 (Scopus)

抄録

Regional distribution of orexin-A-like immunoreactivity in the human brain and pituitary, and the presence of orexin-A-like immunoreactivity in the tumor tissues of pheochromocytomas, ganglioneuroblastomas and neuroblastomas were studied by radioimmunoassay. Expression of orexin mRNA was studied by reverse transcriptase polymerase chain reaction (PCR) method. Orexin-A-like immunoreactivity was detected in every region of human brain, but not in the pituitary. The highest concentration of orexin-A-like immunoreactivity in the human brain was found in hypothalamus (17.8 ± 4.3 pmol/g wet weight, mean ± SEM, n = 7), followed by thalamus, medulla oblongata, and pons. Orexin-A-like immunoreactivity was detected in the tumor tissues of ganglioneuroblastoma and neuroblastoma, but not in the tumor tissues of pheochromocytoma. Reverse phase high performance liquid chromatographic analyses of the orexin-A-like immunoreactivity in the human brain extracts and neuroblastoma extracts showed a single immunoreactive peak, which was eluted in an identical position to synthetic human orexin-A. Orexin mRNA was expressed in the hypothalamus and in the tumor tissues of ganglioneuroblastoma and neuroblastoma. These findings suggest that orexin-A is produced in the hypothalamus and transported to various brain regions via axons. In addition, this study has shown for the first time the production of orexin-A by ganglioneuroblastomas and neuroblastomas. Copyright (C) 2000 Elsevier Science Inc.

本文言語English
ページ(範囲)565-570
ページ数6
ジャーナルPeptides
21
4
DOI
出版ステータスPublished - 2000 4

ASJC Scopus subject areas

  • 生化学
  • 生理学
  • 内分泌学
  • 細胞および分子神経科学

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