Oral MucoRice expressing double-mutant cholera toxin A and B subunits induces toxin-specific neutralising immunity

Yoshikazu Yuki, Daisuke Tokuhara, Tomonori Nochi, Hiroshi Yasuda, Mio Mejima, Shiho Kurokawa, Yuko Takahashi, Nobuhiro Kataoka, Ushio Nakanishi, Yukari Hagiwara, Kohtaro Fujihashi, Fumio Takaiwa, Hiroshi Kiyono

研究成果: Article査読

36 被引用数 (Scopus)

抄録

Rice-expressed cholera toxin B (CTB) subunit is a cold-chain-free oral vaccine that effectively induces enterotoxin-neutralising immunity. We created another rice-based vaccine, MucoRice, expressing nontoxic double-mutant cholera toxin (dmCT) with CTA and CTB subunits. Western-blot analysis suggested that MucoRice-dmCT had the shape of a multicomponent vaccine. Oral administration of MucoRice-dmCT induced CTB- but not CTA-specific serum IgG and mucosal IgA antibodies, generating protective immunity against cholera toxin without inducing rice-protein-specific antibody responses. The potency of MucoRice-dmCT was equal to that of MucoRice-CTB vaccine. MucoRice has the potential to be used as a safe multicomponent vaccine expression system.

本文言語English
ページ(範囲)5982-5988
ページ数7
ジャーナルVaccine
27
43
DOI
出版ステータスPublished - 2009 10 9
外部発表はい

ASJC Scopus subject areas

  • 分子医療
  • 免疫学および微生物学(全般)
  • 獣医学(全般)
  • 公衆衛生学、環境および労働衛生
  • 感染症

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