Optogenetic manipulation of neural and non-neural functions

Hiromu Yawo, Toshifumi Asano, Seiichiro Sakai, Toru Ishizuka

    研究成果: Review article査読

    39 被引用数 (Scopus)

    抄録

    Optogenetic manipulation of the neuronal activity enables one to analyze the neuronal network both in vivo and in vitro with precise spatio-temporal resolution. Channelrhodopsins (ChRs) are light-sensitive cation channels that depolarize the cell membrane, whereas halorhodopsins and archaerhodopsins are light-sensitive Cl- and H+ transporters, respectively, that hyperpolarize it when exogenously expressed. The cause-effect relationship between a neuron and its function in the brain is thus bi-directionally investigated with evidence of necessity and sufficiency. In this review we discuss the potential of optogenetics with a focus on three major requirements for its application: (i) selection of the light-sensitive proteins optimal for optogenetic investigation, (ii) targeted expression of these selected proteins in a specific group of neurons, and (iii) targeted irradiation with high spatiotemporal resolution. We also discuss recent progress in the application of optogenetics to studies of non-neural cells such as glial cells, cardiac and skeletal myocytes. In combination with stem cell technology, optogenetics may be key to successful research using embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) derived from human patients through optical regulation of differentiation-maturation, through optical manipulation of tissue transplants and, furthermore, through facilitating survival and integration of transplants. Development, Growth & Differentiation

    本文言語English
    ページ(範囲)474-490
    ページ数17
    ジャーナルDevelopment Growth and Differentiation
    55
    4
    DOI
    出版ステータスPublished - 2013 5

    ASJC Scopus subject areas

    • Developmental Biology
    • Cell Biology

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