TY - JOUR
T1 - Optimization of operating parameters for xylose reductase separation through ultrafiltration membrane using response surface methodology
AU - Krishnan, Santhana
AU - Suzana, B. Noor
AU - Wahid, Zularisam Abdul
AU - Nasrullah, Mohd
AU - Abdul Munaim, Mimi Sakinah
AU - Din, Mohd Fadhil Bin MD
AU - Taib, Shazwin Mat
AU - Li, Yu You
N1 - Funding Information:
Authors would like to thank Universiti Teknologi Malaysia and AUN/SEED-Net and JICA for the financial support. This work was supported by PDRU Grant- Vot No. Q.J130000.21A2.04E53 , LRGS Grant, Vote MRUN/ F201/2019/5 , Collaborative Research Program for Common Regional Issues (CRC) Grant, Vote: R.J130000.7317.4B189 , The Hitachi Global Foundation , and UTM Matching Grant, Vote: Q.J130000.3017.00M90 .
Funding Information:
Authors would like to thank Universiti Teknologi Malaysia and AUN/SEED-Net and JICA for the financial support. This work was supported by PDRU Grant- Vot No. Q.J130000.21A2.04E53, LRGS Grant, Vote MRUN/ F201/2019/5, Collaborative Research Program for Common Regional Issues (CRC) Grant, Vote: R.J130000.7317.4B189, The Hitachi Global Foundation, and UTM Matching Grant, Vote: Q.J130000.3017.00M90.
Publisher Copyright:
© 2020
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/9
Y1 - 2020/9
N2 - The application of the xylose reductase (XR) enzyme in the development of biotechnology demands an efficient and large scale enzyme separation technique. The aim of this present work was to optimize xylose reductase (XR) purification process through ultrafiltration membrane (UF) technology using Central composite design (CCD) of response surface methods (RSM). The three effective parameters analyzed were filtration time (0–100), transmembrane pressure (TMP) (1–1.6 bar), cross flow velocity (CFV) (0.52–1.2 cm/s−1) and its combined effect to obtain high flux with less possibility of membrane fouling. Experimental studies revealed that the best range for optimization process for filtration time, operational transmembrane pressure and cross flow velocity was 30 min, 1.4 bars and 1.06 cm/s, respectively as these conditions yielded the highest membrane permeability (56.03 Lm-2h-1 bar−1) and xylitol content (15.49 g/l). According to the analysis of variance (ANOVA), the p-value (<0.0001) indicated the designed model was highly significant. The error percentage between the actual and predicted value for membrane permeability and xylitol amount (2.21 % and 4.85 % respectively), which both were found to be close to the predicted values. The verification experiments gave membrane actual permeability of 57.3 Lm-2h-1 bar−1 and 16.29 g/l of xylitol production, thus indicating that the successfully developed model to predict the response.
AB - The application of the xylose reductase (XR) enzyme in the development of biotechnology demands an efficient and large scale enzyme separation technique. The aim of this present work was to optimize xylose reductase (XR) purification process through ultrafiltration membrane (UF) technology using Central composite design (CCD) of response surface methods (RSM). The three effective parameters analyzed were filtration time (0–100), transmembrane pressure (TMP) (1–1.6 bar), cross flow velocity (CFV) (0.52–1.2 cm/s−1) and its combined effect to obtain high flux with less possibility of membrane fouling. Experimental studies revealed that the best range for optimization process for filtration time, operational transmembrane pressure and cross flow velocity was 30 min, 1.4 bars and 1.06 cm/s, respectively as these conditions yielded the highest membrane permeability (56.03 Lm-2h-1 bar−1) and xylitol content (15.49 g/l). According to the analysis of variance (ANOVA), the p-value (<0.0001) indicated the designed model was highly significant. The error percentage between the actual and predicted value for membrane permeability and xylitol amount (2.21 % and 4.85 % respectively), which both were found to be close to the predicted values. The verification experiments gave membrane actual permeability of 57.3 Lm-2h-1 bar−1 and 16.29 g/l of xylitol production, thus indicating that the successfully developed model to predict the response.
KW - Central composite design (CCD)
KW - Cross flow technique
KW - Ultrafiltration (UF)
KW - Xylose reductase (XR)
UR - http://www.scopus.com/inward/record.url?scp=85087475801&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85087475801&partnerID=8YFLogxK
U2 - 10.1016/j.btre.2020.e00498
DO - 10.1016/j.btre.2020.e00498
M3 - Article
AN - SCOPUS:85087475801
VL - 27
JO - Biotechnology Reports
JF - Biotechnology Reports
SN - 2215-017X
M1 - e00498
ER -