Optimization of a method for the simultaneous extraction of polar and non-polar oxylipin metabolites, dna, rna, small rna, and protein from a single small tissue sample

Yu Hasegawa, Yurika Otoki, Shannon McClorry, Laurynne C. Coates, Rachel L. Lombardi, Ameer Y. Taha, Carolyn M. Slupsky

研究成果: Article査読

1 被引用数 (Scopus)

抄録

A more comprehensive picture of tissue biology can be obtained through the application and integration of multiple omic technologies. However, the common challenge in working with a precious sample is having a sample too small to separately extract analytes of interest for each experiment. Considering the high heterogeneity that can be present in a single tissue sample, extracting all biomolecules from a single and undivided tissue is preferable because it allows direct comparison of results. Here, we combined a modified Folch extraction method with DNA, RNA, small RNA, and protein extraction using two commercial kits, which allowed us to extract polar metabolites and non-polar oxylipin metabolites, DNA, RNA, small RNA, and protein simultaneously from a small tissue sample. The method was validated in terms of quantity and quality of analytes for downstream analyses.

本文言語English
論文番号61
ページ(範囲)1-14
ページ数14
ジャーナルMethods and Protocols
3
3
DOI
出版ステータスPublished - 2020 9

ASJC Scopus subject areas

  • バイオテクノロジー
  • 構造生物学
  • 生化学、遺伝学、分子生物学(その他)

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