TY - JOUR
T1 - Optimization of 1-deoxynojirimycin extraction from mulberry leaves by using response surface methodology
AU - Vichasilp, Chaluntorn
AU - Nakagawa, Kiyotaka
AU - Sookwong, Phumon
AU - Suzuki, Yasuyo
AU - Kimura, Fumiko
AU - Higuchi, Ohki
AU - Miyazawa, Teruo
N1 - Funding Information:
This work was supported by the Supporting Program for Creating University Ventures, Japan Science and Technology Agency (to T. M.).
PY - 2009
Y1 - 2009
N2 - Mulberry 1-deoxynojirimycin (DNJ, a potent α-glycosidase inhibitor) has therapeutic potency against diabetes mellitus. However, the amount of DNJ in mulberry leaves is low (about 0.1%), and a more effective extraction method is needed. Ultrasound-assisted extraction (UAE) was applied in this study for mulberry DNJ extraction, and five factors, the percentage of ethanol in the extraction solvent (x1), ratio of the extraction solvent to mulberry sample (x2), ultrasonic power (x3), extraction temperature (x4) and extraction time (x5), were investigated by fractional factorial 2(5-1) design (FFD) to obtain the optimum extraction efficiency (DNJ yield, Y1) and extraction productivity (total yield, Y2). The results showed that x2, x 3 and x5 had significant impact on Y1 and Y2, and were further optimized by response surface methodology (RSM). Under the optimized conditions (x2, x3 and x5 of 7 ml/g, 180 W and 260 s, respectively), DNJ-enriched powder (0.8%) was produced with high extraction efficiency (98%) and productivity (20%), enabling this product to be used for nutraceutical purposes.
AB - Mulberry 1-deoxynojirimycin (DNJ, a potent α-glycosidase inhibitor) has therapeutic potency against diabetes mellitus. However, the amount of DNJ in mulberry leaves is low (about 0.1%), and a more effective extraction method is needed. Ultrasound-assisted extraction (UAE) was applied in this study for mulberry DNJ extraction, and five factors, the percentage of ethanol in the extraction solvent (x1), ratio of the extraction solvent to mulberry sample (x2), ultrasonic power (x3), extraction temperature (x4) and extraction time (x5), were investigated by fractional factorial 2(5-1) design (FFD) to obtain the optimum extraction efficiency (DNJ yield, Y1) and extraction productivity (total yield, Y2). The results showed that x2, x 3 and x5 had significant impact on Y1 and Y2, and were further optimized by response surface methodology (RSM). Under the optimized conditions (x2, x3 and x5 of 7 ml/g, 180 W and 260 s, respectively), DNJ-enriched powder (0.8%) was produced with high extraction efficiency (98%) and productivity (20%), enabling this product to be used for nutraceutical purposes.
KW - 1-Deoxynojirimycin (DNJ)
KW - Mulberry
KW - Response surface methodology (RSM)
KW - Ultrasound-assisted extraction (UAE)
UR - http://www.scopus.com/inward/record.url?scp=74349130206&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=74349130206&partnerID=8YFLogxK
U2 - 10.1271/bbb.90543
DO - 10.1271/bbb.90543
M3 - Article
C2 - 19966480
AN - SCOPUS:74349130206
VL - 73
SP - 2684
EP - 2689
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 12
ER -
