Optical detection of photo-activated protein using Brewster angle microscopy

Y. Li, S. Gorelik, N. Tomczak, H. Y. Song, M. J. Lear, J. Hobley

研究成果: Conference contribution

抄録

Bovine serum albumin (BSA) was space selectively immobilized onto a quartz substrate using photo-reactions initiated by pulsed laser irradiation of benzophenone derivatives tethered by silane coupling to the quartz. The excitation of the benzophenone with 266nm light yields the π-π* triplet state with a quantum yield of 1. This triplet state then reacts with BSA in buffer solution in contact with the modified quartz. The triplet state may form a ketyl radical by reaction with the solution or it may abstract a proton directly from the BSA becoming covalently tethered to the surface. The entire deposition process was monitored in real time, insitu using a Brewster angle microscope (BAM).

本文言語English
ホスト出版物のタイトル2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008
DOI
出版ステータスPublished - 2008 12月 1
イベント2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008 - Singapore, Singapore
継続期間: 2008 12月 82008 12月 11

出版物シリーズ

名前2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008

Conference

Conference2008 IEEE PhotonicsGlobal at Singapore, IPGC 2008
国/地域Singapore
CitySingapore
Period08/12/808/12/11

ASJC Scopus subject areas

  • コンピュータ ネットワークおよび通信
  • 電子工学および電気工学

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