On-chip transformation of bacteria

Kuniaki Nagamine, Shiho Onodera, Yu Suke Torisawa, Tomoyuki Yasukawa, Hitoshi Shiku, Tomokazu Matsue

研究成果: Article査読

31 被引用数 (Scopus)

抄録

On-chip transformation of Escherichia coli cells was accomplished for the first time using a microbial array chip. The continuous E. coli transformation procedures were performed on a chip in which the microcompartment was composed of PDMS microfluidic channels and a silicon substrate predeposited with different plasmid DNAs. The PDMS microfluidic device enabled the parallel transformation of E. coli cells with various plasmid DNAs by separating each transformation area. The phenotypic differences reflecting different plasmid DNAs were identified by various approaches such as colorimetry, fluorometry, and electrochemical methods. This microbial array chip could become a versatile tool for many cell biological applications.

本文言語English
ページ(範囲)4278-4281
ページ数4
ジャーナルAnalytical Chemistry
77
13
DOI
出版ステータスPublished - 2005 7月 1

ASJC Scopus subject areas

  • 分析化学

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