Oligomerization of a modular ribozyme assembly of which is controlled by a programmable RNA–RNA interface between two structural modules

Ryusei Tsuruga, Narumi Uehara, Yuki Suzuki, Hiroyuki Furuta, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, Yoshiya Ikawa

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Bimolecular ribozymes derived by physical dissection of unimolecular ribozymes consisting of two structural modules are promising platforms for the design and construction of assembled RNA nanostructures. Unit RNAs to be assembled intermolecularly into one-dimensional (1D) oligomers are designed by reconnecting the two structural modules in a manner different from the parent ribozymes. This strategy was applied to the Tetrahymena group I ribozyme. We constructed 1D ribozyme oligomers the assembly of which was observed by atomic force microscopy (AFM) and also controlled rationally to design a heterooctamer by differentiating the interface between the two modules.

本文言語English
ページ(範囲)410-415
ページ数6
ジャーナルJournal of Bioscience and Bioengineering
128
4
DOI
出版ステータスPublished - 2019 10
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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