Olfactory receptors are expressed in pancreatic β-cells and promote glucose-stimulated insulin secretion

Yuichiro Munakata, Tetsuya Yamada, Junta Imai, Kei Takahashi, Sohei Tsukita, Yuta Shirai, Shinjiro Kodama, Yoichiro Asai, Takashi Sugisawa, Yumiko Chiba, Keizo Kaneko, Kenji Uno, Shojiro Sawada, Hiroyasu Hatakeyama, Makoto Kanzaki, Jun Ichi Miyazaki, Yoshitomo Oka, Hideki Katagiri

研究成果: Article査読

22 被引用数 (Scopus)


Olfactory receptors (ORs) mediate olfactory chemo-sensation in OR neurons. Herein, we have demonstrated that the OR chemo-sensing machinery functions in pancreatic β-cells and modulates insulin secretion. First, we found several OR isoforms, including OLFR15 and OLFR821, to be expressed in pancreatic islets and a β-cell line, MIN6. Immunostaining revealed OLFR15 and OLFR821 to be uniformly expressed in pancreatic β-cells. In addition, mRNAs of Olfr15 and Olfr821 were detected in single MIN6 cells. These results indicate that multiple ORs are simultaneously expressed in individual β-cells. Octanoic acid, which is a medium-chain fatty acid contained in food and reportedly interacts with OLFR15, potentiated glucose-stimulated insulin secretion (GSIS), thereby improving glucose tolerance in vivo. GSIS potentiation by octanoic acid was confirmed in isolated pancreatic islets and MIN6 cells and was blocked by OLFR15 knockdown. While Gαolf expression was not detectable in β-cells, experiments using inhibitors and siRNA revealed that the pathway dependent on phospholipase C-inositol triphosphate, rather than cAMP-protein kinase A, mediates GSIS potentiation via OLFR15. These findings suggest that the OR system in pancreatic β-cells has a chemo-sensor function allowing recognition of environmental substances obtained from food, and potentiates insulin secretion in a cell-autonomous manner, thereby modulating systemic glucose metabolism.

ジャーナルScientific reports
出版ステータスPublished - 2018 12 1

ASJC Scopus subject areas

  • 一般


「Olfactory receptors are expressed in pancreatic β-cells and promote glucose-stimulated insulin secretion」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。