Oil-free hyaluronic acid matrix for serial femtosecond crystallography

Michihiro Sugahara, Changyong Song, Mamoru Suzuki, Tetsuya Masuda, Shigeyuki Inoue, Takanori Nakane, Fumiaki Yumoto, Eriko Nango, Rie Tanaka, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, Keiji Numata, So Iwata

研究成果: Article査読

29 被引用数 (Scopus)

抄録

The grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we introduce a grease-free, water-based hyaluronic acid matrix. Applications for proteinase K and lysozyme proteins were able to produce electron density maps at 2.3-Å resolution.

本文言語English
論文番号24484
ジャーナルScientific reports
6
DOI
出版ステータスPublished - 2016 4 18
外部発表はい

ASJC Scopus subject areas

  • General

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