Occurrence of Two Types of Ca2+‐Dependent Protein Kinases in the Cytosol Fraction of the Brain

E. Miyamoto, K. Fukunaga, K. Matsui, Y. Iwasa

研究成果: Article査読

39 被引用数 (Scopus)

抄録

Abstract— Two types of Ca2+‐dependent protein kinases were demonstrated and partially purified from the cytosol fraction of rat brain by DEAE‐cellulose, Sephadex G‐200, and calmodulin‐affinity column chromatography, using endogenous proteins and chicken gizzard myosin light chains as substrates. The molecular weights of the enzymes were 88,000 (peak I) and 120,000 (peak II) on gel filtration. Peak I had no affinity for calmodulin, whereas peak II had a high affinity for it, with a Ka value of 16.7 nm. The Ka values of peaks I and II for Ca2+ were 2.4 and 1.6 μm, respectively.

本文言語English
ページ(範囲)1324-1330
ページ数7
ジャーナルJournal of Neurochemistry
37
5
DOI
出版ステータスPublished - 1981 11
外部発表はい

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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