Observation of the dynamics of live cardiomyocytes through a free-running scanning near-field optical microscopy setup

Ruggero Micheletto, Morgan Denyer, Martin Scholl, Ken Nakajima, Andreas Offenhauser, Masahiko Hara, Wolfgang Knoll

    研究成果: Article査読

    21 被引用数 (Scopus)

    抄録

    We report the observation of live-cell dynamics by noncontact scanning near-field optical microscopy (SNOM) modified to work with living biological samples that are fully immersed in liquid. We did not use the SNOM setup in strictly near-field conditions (we used 1-mm constant-height mode); however, we could examine the dynamics of rhythmically beating cardiac myocytes in culture with extremely high vertical sensitivity below the nanometric range. We could halt scans at any point to record localized contraction profiles of the cell membrane. We show that the contractions of the organisms changed shape dramatically within adjacent areas. We believe that the spatial dependency of the contractions arises because of the measurement system’s ability to resolve the behavior of individual submembrane actin bundles. Our results, combining imaging and real-time recording in localized areas, reveal a new, to our knowledge, noninvasive method for using SNOM setups for studying the dynamics of live biological samples.

    本文言語English
    ページ(範囲)6648-6652
    ページ数5
    ジャーナルApplied optics
    38
    31
    DOI
    出版ステータスPublished - 1999 10 20

    ASJC Scopus subject areas

    • 原子分子物理学および光学
    • 工学(その他)
    • 電子工学および電気工学

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