TY - JOUR
T1 - Observation of the dynamics of live cardiomyocytes through a free-running scanning near-field optical microscopy setup
AU - Micheletto, Ruggero
AU - Denyer, Morgan
AU - Scholl, Martin
AU - Nakajima, Ken
AU - Offenhauser, Andreas
AU - Hara, Masahiko
AU - Knoll, Wolfgang
PY - 1999/10/20
Y1 - 1999/10/20
N2 - We report the observation of live-cell dynamics by noncontact scanning near-field optical microscopy (SNOM) modified to work with living biological samples that are fully immersed in liquid. We did not use the SNOM setup in strictly near-field conditions (we used 1-mm constant-height mode); however, we could examine the dynamics of rhythmically beating cardiac myocytes in culture with extremely high vertical sensitivity below the nanometric range. We could halt scans at any point to record localized contraction profiles of the cell membrane. We show that the contractions of the organisms changed shape dramatically within adjacent areas. We believe that the spatial dependency of the contractions arises because of the measurement system’s ability to resolve the behavior of individual submembrane actin bundles. Our results, combining imaging and real-time recording in localized areas, reveal a new, to our knowledge, noninvasive method for using SNOM setups for studying the dynamics of live biological samples.
AB - We report the observation of live-cell dynamics by noncontact scanning near-field optical microscopy (SNOM) modified to work with living biological samples that are fully immersed in liquid. We did not use the SNOM setup in strictly near-field conditions (we used 1-mm constant-height mode); however, we could examine the dynamics of rhythmically beating cardiac myocytes in culture with extremely high vertical sensitivity below the nanometric range. We could halt scans at any point to record localized contraction profiles of the cell membrane. We show that the contractions of the organisms changed shape dramatically within adjacent areas. We believe that the spatial dependency of the contractions arises because of the measurement system’s ability to resolve the behavior of individual submembrane actin bundles. Our results, combining imaging and real-time recording in localized areas, reveal a new, to our knowledge, noninvasive method for using SNOM setups for studying the dynamics of live biological samples.
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U2 - 10.1364/AO.38.006648
DO - 10.1364/AO.38.006648
M3 - Article
C2 - 18324201
AN - SCOPUS:0000629496
VL - 38
SP - 6648
EP - 6652
JO - Applied Optics
JF - Applied Optics
SN - 0003-6935
IS - 31
ER -