The rice-stylo (Stylosanthes guianensis) relay-intercropping system in paddy fields has previously been shown to increase agricultural productivity in Northeast Thailand, but successive relay-intercropping conducted without irrigation and fertilization during the stylo cropping has resulted in reduced production of stylo. The aim of this study was to determine the effect of nutrient deficiency on the stylo production. The experiment was conducted using pots to control fertilization accurately. The results showed that, after three successive relayintercroppings, the soil was severely deficient in sulfur, phosphorus, and molybdenum. Sulfate application to the soil after the successive relay-intercroppings was suggested to improve stylo production. We recommend the use of a rain-fed shallow, favorable subecosystem as a field for stylo production in relay-intercropping and the stylo production with a cycle of several years.
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