Nucleotide sequence of the tobacco mosaic virus (tomato strain) genome and comparison with the common strain genome

Takeshi Ohno, Midori Aoyagi, Yuji Yamanashi, Hiraku Saito, Shuntaro Ikawa, Tetsuo Meshi, Yoshimi Okada

研究成果: Article

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The sequence of about 4,500 nucleotides of the internal part of tobacco mosaic virus (TMV)tomato strain (L) RNA has been newly determined using cloned cDNAs. Together with the previously determined partial sequences at both ends, the entire sequence of the 6,384 nucleotide genome has been completed. The 130K (1,115 amino acids), 180K (1,615 amino acids), 30K (263 amino acids) and coat protein (158 amino acids) cistrons are located at residues 72-3442, 72-4922, 4906-5700, and 5703-6182 on the genome, respectively. Sequence polymorphism was not observed except for heterogeneity in the length of the A cluster near the 3' end. The homology of the nucleotide sequences of TMV-L and TMV-vulgare, a common strain, is about 80%on average. Remarkable differences between them were found in a part of the N-terminal portion of the 130K/180K protein and the C-terminal portion of the 30K protein. A new method for cDNA cloning was developed by which the cDNA of the 5'-terminus of viral RNA can be cloned efficiently.

元の言語English
ページ(範囲)1915-1923
ページ数9
ジャーナルJournal of biochemistry
96
発行部数6
DOI
出版物ステータスPublished - 1984 10
外部発表Yes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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