Nucleotide sequence of calf prorennin cDNA cloned in Escherichia coli

Katsuhiko Nishimori, Yoshiyuki Kawaguchi, Makoto Hidaka, Takeshi Uozumi, Teruhiko Beppu

研究成果: Article査読

30 被引用数 (Scopus)

抄録

The nucleotide sequence of prorennin (prochymosin) cDNA cloned in E. coli was determined by the technique of Maxam and Gilbert. The longest prorennin cDNA insert in pTACR1 contained the putative signal sequence and the coding sequence for the peptide from the 1st amino acid, Ala (NH2 terminal), to the 296th, Ser, and the other clone pTACR9 contained the coding sequence from the 258th, Asp, to the 365th, Ile (COOH terminal), and the TGA termination codon followed by the 3′-untranslated region. Thus, the whole coding sequence for prorennin was obtained in the pair of pTACR1 and pTACR9.

本文言語English
ページ(範囲)1085-1088
ページ数4
ジャーナルJournal of biochemistry
91
3
DOI
出版ステータスPublished - 1982 1月 1
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

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