Novel methylcellulose-immobilized cation-exchange precolumn for on-line enrichment of cationic drugs in plasma

Eiichi Yamamoto, Takahisa Sakaguchi, Takashi Kajima, Nariyasu Mano, Naoki Asakawa

研究成果: Article査読

28 被引用数 (Scopus)

抄録

We developed a novel methylcellulose-immobilized strong cation-exchange (MC-SCX) precolumn for direct analysis of drugs in plasma. MC-SCX consists of silica gel with a methylcellulose outer-surface and a 2-(4-sulfophenyl) ethyl phase inner-surface. The MC-SCX precolumn was evaluated by direct analysis using pyridoxine, atenolol and sulpiride spiked in plasma, using a column-switching HPLC system. Each drug was retained and enriched on MC-SCX using an acidic mobile phase, which resulted in good linearity, sufficient reproducibility, intra- and inter day precision, and accuracy in analytical ion-pair LC with trifluoroacetic acid. The analytical methods for model drugs were applied to pharmacokinetics of atenolol and sulpiride in rats.

本文言語English
ページ(範囲)327-334
ページ数8
ジャーナルJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
807
2
DOI
出版ステータスPublished - 2004 8 5

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 臨床生化学
  • 細胞生物学

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