TY - CHAP
T1 - Novel intermolecular surface force unveils the driving force of the actomyosin system
AU - Suzuki, Makoto
AU - Mogami, George
AU - Watanabe, Takahiro
AU - Matubayasi, Nobuyuki
PY - 2018/5/7
Y1 - 2018/5/7
N2 - In this chapter, we discuss the role of water in actomyosin-force generation. We have been investigating the hydration properties of ions, organic molecules, and proteins. These studies revealed that actin filaments (F-actin) are surrounded by a hyper-mobile water (HMW) layer and restrained water layer, while myosin subfragment 1 (S1) has only a typical restrained hydration layer. The understanding of the physicochemical properties of HMW has been greatly advanced by recent theoretical studies on statistical mechanics and solution chemistry. To explain the mechanism of force generation of actomyosin using ATP hydrolysis, we propose a driving force hypothesis based on novel intermolecular surface force. This hypothesis is consistent with the reported biochemical kinetics and thermodynamic parameters for the primary reaction steps. The gradient field of solvation free energy of S1 is generated in close proximity to F-actin.
AB - In this chapter, we discuss the role of water in actomyosin-force generation. We have been investigating the hydration properties of ions, organic molecules, and proteins. These studies revealed that actin filaments (F-actin) are surrounded by a hyper-mobile water (HMW) layer and restrained water layer, while myosin subfragment 1 (S1) has only a typical restrained hydration layer. The understanding of the physicochemical properties of HMW has been greatly advanced by recent theoretical studies on statistical mechanics and solution chemistry. To explain the mechanism of force generation of actomyosin using ATP hydrolysis, we propose a driving force hypothesis based on novel intermolecular surface force. This hypothesis is consistent with the reported biochemical kinetics and thermodynamic parameters for the primary reaction steps. The gradient field of solvation free energy of S1 is generated in close proximity to F-actin.
KW - ATP hydrolysis
KW - Electric field effect
KW - Hydration free energy motor protein
KW - Protein-water interaction
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U2 - 10.1007/978-981-10-8459-1_16
DO - 10.1007/978-981-10-8459-1_16
M3 - Chapter
AN - SCOPUS:85053958757
SN - 9789811084584
SP - 257
EP - 274
BT - The Role of Water in ATP Hydrolysis Energy Transduction by Protein Machinery
PB - Springer Singapore
ER -