Novel cell culture substrates based on micro-porous films of amphiphilic polymers

Honeycomb-like micro-porous films (honeycomb films) can be fabricated by casting dilute solutions of amphiphilic polymers on solid supports in humid atmosphere. We have been studying how the honeycomb films can be used as artificial extracellular matrices. Here we introduce two fabrication methods for the honeycomb films which provide different surface coverage to solid substrates. The honeycomb films which can make the solid substrates appear from their micro pores were utilized for the micro-patterning of cell culture substrates. Hepatocytes recognized the difference in the surface coverage of solid supports.