Nonradioactive assay for adenosine 5'-phosphosulfate sulfotransferase using reversed-phase ion-pair high-performance liquid chromatography

N. Kanno, E. Nagahisa, M. Sato, Y. Sato

    研究成果: Article査読

    6 被引用数 (Scopus)

    抄録

    A nonradioactive and simple method for the assay of adenosine 5'-phosphosulfate sulfotransferase (APSSTase) is described. The method is based upon the quantitative measurement of the substrate adenosine 5'-phosphosulfate and/or product AMP by ion-pair reversed-phase high-performance liquid chromatography on a C18 column. Isocratic elution renders the method simple and fast. This method is standardized for partially purified APSSTase from the marine red macroalgia Porphyra yezoensis.

    本文言語English
    ページ(範囲)47-55
    ページ数9
    ジャーナルBiochemistry and Molecular Biology International
    29
    1
    出版ステータスPublished - 1993 1月 1

    ASJC Scopus subject areas

    • 生化学
    • 遺伝学
    • 分子生物学

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