Nonradioactive assay for adenosine 5'-phosphosulfate sulfotransferase using reversed-phase ion-pair high-performance liquid chromatography

N. Kanno; E. Nagahisa; M. Sato; Y. Sato

Nonradioactive assay for adenosine 5'-phosphosulfate sulfotransferase using reversed-phase ion-pair high-performance liquid chromatography

N. Kanno, E. Nagahisa, M. Sato, Y. Sato

研究成果: Article › 査読

抄録

A nonradioactive and simple method for the assay of adenosine 5'-phosphosulfate sulfotransferase (APSSTase) is described. The method is based upon the quantitative measurement of the substrate adenosine 5'-phosphosulfate and/or product AMP by ion-pair reversed-phase high-performance liquid chromatography on a C₁₈ column. Isocratic elution renders the method simple and fast. This method is standardized for partially purified APSSTase from the marine red macroalgia Porphyra yezoensis.

本文言語	English
ページ（範囲）	47-55
ページ数	9
ジャーナル	Biochemistry and Molecular Biology International
巻	29
号	1
出版ステータス	Published - 1993 1月 1

ASJC Scopus subject areas

生化学
遺伝学
分子生物学

UN SDG

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引用スタイル

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abstract = "A nonradioactive and simple method for the assay of adenosine 5'-phosphosulfate sulfotransferase (APSSTase) is described. The method is based upon the quantitative measurement of the substrate adenosine 5'-phosphosulfate and/or product AMP by ion-pair reversed-phase high-performance liquid chromatography on a C18 column. Isocratic elution renders the method simple and fast. This method is standardized for partially purified APSSTase from the marine red macroalgia Porphyra yezoensis.",

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N2 - A nonradioactive and simple method for the assay of adenosine 5'-phosphosulfate sulfotransferase (APSSTase) is described. The method is based upon the quantitative measurement of the substrate adenosine 5'-phosphosulfate and/or product AMP by ion-pair reversed-phase high-performance liquid chromatography on a C18 column. Isocratic elution renders the method simple and fast. This method is standardized for partially purified APSSTase from the marine red macroalgia Porphyra yezoensis.

AB - A nonradioactive and simple method for the assay of adenosine 5'-phosphosulfate sulfotransferase (APSSTase) is described. The method is based upon the quantitative measurement of the substrate adenosine 5'-phosphosulfate and/or product AMP by ion-pair reversed-phase high-performance liquid chromatography on a C18 column. Isocratic elution renders the method simple and fast. This method is standardized for partially purified APSSTase from the marine red macroalgia Porphyra yezoensis.

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