Noncompetitive Fluorescence Polarization Immunoassay for Protein Determination

Mao Fukuyama, Ayano Nakamura, Keine Nishiyama, Ayuko Imai, Manabu Tokeshi, Koji Shigemura, Akihide Hibara

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Fluorescent polarization immunoassay (FPIA) is a single-step immunoassay method that is applicable to point-of-care testing; however, its applicability to large biomolecules has been restricted because ordinary FPIA is a competitive assay. Here, we report a noncompetitive FPIA using the variable domain from the heavy chain of a camelid antibody (VHH antibody). FPIA with VHH was successfully used to quantitate rabbit immunoglobulin G (IgG) and demonstrated a wider response range than that observed with antibody-binding (Fab) fragment. Then, using a portable FPIA instrument, a VHH-based immunoassay of human IgG in a human serum certified reference material was demonstrated.

本文言語English
ページ(範囲)14393-14397
ページ数5
ジャーナルAnalytical Chemistry
92
21
DOI
出版ステータスPublished - 2020 11 3

ASJC Scopus subject areas

  • Analytical Chemistry

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