New epidermal keratin genes from Xenopus laevis: Hormonal and regional regulation of their expression during anuran skin metamorphosis

Yusuke Watanabe, Hisao Kobayashi, Ken ichi Suzuki, Kaori Kotani, Katsutoshi Yoshizato

研究成果: Article査読

38 被引用数 (Scopus)

抄録

Xenopus larval keratin (XLK) was isolated by gel electrophoresis of proteins of tadpole skin. Screening of an expression cDNA library of tail tissues by specific polyclonal antibodies against XLK produced XLK cDNA (xlk). Its complete nucleotide and predicted amino acid sequences revealed that XLK was a new member of type II keratin. Screening of a cDNA library of adult Xenopus skin using an oligonucleotide probe which had been designed from well-conserved N-terminal amino acid sequences of the rod domain of type I keratin produced two cDNAs, xak-a and xak-b, which were found to be new members of type I keratin gene. Northern blot analysis showed that xlk was expressed exclusively in the larval skin whereas xak-a and xak-b were expressed exclusively in the adult skin. Their expression level was regulated in a region- and metamorphic stage-dependent manner during larval skin development. mRNA in situ hybridization experiments identified the cells that expressed xlk, and xak-a and xak-b as larva-specific epidermal cells (skein cells and basal cells), and adult suprabasal epidermal cells, respectively. These three genes were found to be late responsive to thyroid hormone. Phylogenetic relationships of these keratins with known ones are discussed.

本文言語English
ページ(範囲)339-350
ページ数12
ジャーナルBiochimica et Biophysica Acta - Gene Structure and Expression
1517
3
DOI
出版ステータスPublished - 2001 2 16

ASJC Scopus subject areas

  • 構造生物学
  • 生物理学
  • 生化学
  • 遺伝学

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