Neuropeptide Y inhibits neurogenic inflammation in guinea pig airways

T. Takahashi, M. Ichinose, H. Yamauchi, M. Miura, N. Nakajima, J. Ishikawa, H. Inoue, T. Takishima, K. Shirato

研究成果: Article査読

20 被引用数 (Scopus)


We examined the effect of neuropeptide Y (NPY) on neurogenic airway microvascular leakage. Male Dunkin-Hartley guinea pigs (250-350 g) were anesthetized with urethan (2 g/kg ip). The cervical artery and vein were cannulated for monitoring blood pressure and injecting drugs, respectively. Atropine and propranolol (each 1 mg/kg iv) were administered 30 min before the experiment. After pretreatment with saline (vehicle for NPY) or NPY (1- 100 μg/kg iv), Evans blue dye (30 mg/kg iv) was administered. Then, bilateral vagal nerves were electrically stimulated (5 V, 7 Hz, 5-ms duration for 3 min) to induce airway plasma leakage. Airways were divided into four sections [trachea (Tr), main bronchi, central intrapulmonary airways (IPA), and peripheral IPA] and incubated in formamide (37°C for 16 h). The concentration of Evans blue dye was measured by spectrophotometer. Furthermore, we examined the effect of NPY on exogenous substance P- (0.3 μg/kg iv) induced plasma extravasation. Bilateral vagal stimulation significantly increased leakage of dye in Tr to peripheral IPA. NPY did not affect basal leakage but did significantly inhibit neurogenic plasma extravasation in a dose-dependent manner with maximal inhibitions of 42.3 (Tr), 67.7 (main bronchi), 38.2 (central IPA), and 26.3% (peripheral IPA) at 30 μg/kg. Exogenous substance P-induced plasma extravasation was not inhibited by NPY. We conclude that NPY inhibits neurogenic inflammation by prejunctional inhibition of neuropeptide release from airway sensory nerve terminals.

ジャーナルJournal of Applied Physiology
出版ステータスPublished - 1993

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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