Nek5, a novel substrate for caspase-3, promotes skeletal muscle differentiation by up-regulating caspase activity

Kouhei Shimizu, Tatsuya Sawasaki

研究成果: Article査読

17 被引用数 (Scopus)

抄録

Accumulating evidence suggests that caspase-3-mediated cleavage of protein kinase could be a key event to regulate cell differentiation. In this study, we investigated the role of Nek5 kinase, identified as a novel substrate for caspase-3, in skeletal muscle differentiation. Up-regulation of Nek5 mRNA expression was accompanied by cell differentiation. Myotube formation was promoted in Nek5 expressing cells, and was conversely inhibited in Nek5 knockdown cells. Furthermore, we found that caspase-3 activity, an important factor for myogenic differentiation, was enhanced by Nek5 cleavage. Although caspase-3-cleaved Nek5 partially exerted a promyogenic effect, it tended to induce apoptotic cell death. In summary, our findings suggest that Nek5 promotes myogenic differentiation through up-regulation of caspase activity.

本文言語English
ページ(範囲)2219-2225
ページ数7
ジャーナルFEBS Letters
587
14
DOI
出版ステータスPublished - 2013 7 11

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

フィンガープリント

「Nek5, a novel substrate for caspase-3, promotes skeletal muscle differentiation by up-regulating caspase activity」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル