Native sulfur/chlorine SAD phasing for serial femtosecond crystallography

Takanori Nakane, Changyong Song, Mamoru Suzuki, Eriko Nango, Jun Kobayashi, Tetsuya Masuda, Shigeyuki Inoue, Eiichi Mizohata, Toru Nakatsu, Tomoyuki Tanaka, Rie Tanaka, Tatsuro Shimamura, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, So Iwata, Michihiro Sugahara

研究成果: Article査読

46 被引用数 (Scopus)

抄録

Serial femtosecond crystallography (SFX) allows structures to be determined with minimal radiation damage. However, phasing native crystals in SFX is not very common. Here, the structure determination of native lysozyme from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of sulfur and chlorine at a wavelength of 1.77 Å is successfully demonstrated. This sulfur SAD method can be applied to a wide range of proteins, which will improve the determination of native crystal structures.

本文言語English
ページ(範囲)2519-2525
ページ数7
ジャーナルActa Crystallographica Section D: Biological Crystallography
71
DOI
出版ステータスPublished - 2015
外部発表はい

ASJC Scopus subject areas

  • 構造生物学

フィンガープリント

「Native sulfur/chlorine SAD phasing for serial femtosecond crystallography」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル