Na+- and Cl--Dependent transport of taurine at the blood-brain barrier

Ikumi Tamai, Mizuho Senmaru, Tetsuya Terasaki, Akira Tsuji

研究成果: Article査読

72 被引用数 (Scopus)

抄録

The characteristics of carrier-mediated transport of taurine at the blood-brain barrier (BBB) were studied by using primary cultured bovine brain capillary endothelial cells (BCECs), in situ brain perfusion and brain capillary depletion methods in rats. The uptake of [3H]taurine by cultured cells showed that the active transporter functions on both the luminal and antiluminal membranes of BCECs. The kinetic parameters for the saturable transport of taurine were estimated to be: for the luminal uptake, the Michaelis constant, Kv, was 12.1 ± 0.5 μM, and the maximum uptake rate, Jmax, was 4.32 ± 0.05 nmol/30 min/mg protein; for the antiluminal uptake, Kt was 13.6 ± 2.4 μM and Jmax was 2.81 ± 0.22 nmol/30 min/mg protein. The luminal and antiluminal uptakes of [3H]taurine were each dependent on both Na+ and Cl-. Stoichiometric analyses suggest that two Na+ and one Cl- are associated with the luminal uptake of one taurine molecule. β-Amino acids such as β-alanine and hypotaurine strongly inhibited the uptake of [3H]taurine, whereas α- and γ-amino acids had little or no effect. Furthermore, by in situ brain perfusion and in vivo brain capillary depletion methods, the carrier-mediated transport found by in vitro experiments was confirmed to function for the translocation of the taurine molecule from the vascular space into the brain. From these results, it was concluded that a Na+ and Cl- gradient-dependent transport (uptake) system for taurine exists in both the luminal and the antiluminal membranes of BCECs.

本文言語English
ページ(範囲)1783-1793
ページ数11
ジャーナルBiochemical Pharmacology
50
11
DOI
出版ステータスPublished - 1995 11 27
外部発表はい

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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