Nasopharyngeal-associated lymphoreticular tissue (NALT) immunity: Fimbriae-specific Th1 and Th2 cell-regulated IgA responses for the inhibition of bacterial attachment to epithelial cells and subsequent inflammatory cytokine production

Manabu Yanagita, Takachika Hiroi, Noriko Kitagaki, Shigeyuki Hamada, Ito Hiro-o, Hidetoshi Shimauchi, Shinya Murakami, Hiroshi Okada, Hiroshi Kiyono

研究成果: Article査読

109 被引用数 (Scopus)

抄録

To investigate the antibacterial activity of mucosal Th1 and Th2 immune responses induced nasally and orally, mice were immunized with mucosal vaccine containing fimbrial protein of Porphyromonas gingivalis, a causative agent for a destructive chronic inflammation in the periodontium, and cholera toxin (CT) as mucosal adjuvant. Nasal vaccine containing low doses of fimbriae (10 μg) and CT (1 μg) induced Ag-specific Th1/Th2-type response in CD4+ T cells in mucosal effector tissues, including nasal passage and submandibular glands, which accounted for the generation of Ag-specific IgA- producing cells. In contrast, oral 'immunization required higher amounts of fimbriae and CT for the induction of Ag-specific IgA responses. Fimbriae- specific IgA mAbs generated from submandibular glands of nasally immunized mice inhibited P. gingivalis attachment to and reduced subsequent inflammatory cytokine production from epithelial cells. These findings suggest that nasal vaccination is an effective immunization regimen for the induction of Ag-specific Th1 and Th2 cell-driven IgA immune responses that possess the ability to inhibit bacterial attachment to epithelial cells and subsequent inflammatory cytokine production.

本文言語English
ページ(範囲)3559-3565
ページ数7
ジャーナルJournal of Immunology
162
6
出版ステータスPublished - 1999 3 15

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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