Nanoscale Imaging of Primary Cilia with Scanning Ion Conductance Microscopy

Yuanshu Zhou, Masaki Saito, Takafumi Miyamoto, Pavel Novak, Andrew I. Shevchuk, Yuri E. Korchev, Takeshi Fukuma, Yasufumi Takahashi

研究成果: Article査読

25 被引用数 (Scopus)

抄録

Primary cilia are hair-like sensory organelles whose dimensions and location vary with cell type and culture condition. Herein, we employed scanning ion conductance microscopy (SICM) to visualize the topography of primary cilia from different cell types. By combining SICM with fluorescence imaging, we successfully distinguished between surface cilia that project outward from the cell surface and subsurface cilia that are trapped below it. The nanoscale structure of the ciliary pocket, which cannot be easily identified using a confocal fluorescence microscope, was observed in SICM images. Furthermore, we developed a topographic reconstruction method using current-distance profiles to evaluate the relationship between set point and topographic image and found that a low set point is important for detecting the true topography of a primary cilium using hopping mode SICM.

本文言語English
ページ(範囲)2891-2895
ページ数5
ジャーナルAnalytical Chemistry
90
4
DOI
出版ステータスPublished - 2018 2月 20
外部発表はい

ASJC Scopus subject areas

  • 分析化学

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